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A High Performance Theory for Thermodynamic Study on the Binding of Human Serum Albumin with Erbium Chloride
A thermodynamic study of the interaction between erbium(Ⅲ) chloride (Er3+) and human serum albumin (HAS) was studied at pH=7.0, 27 and 37℃ in phosphate buffer by isothermal titration calorimetry (ITC). The present study reports the thermodynamic parameters that govern HAS-Er3+ interactions. The extended solvation theory was used to reproduce the enthalpies of HAS-Er3+ interactions over the whole range of Er3+ concentrations. The bind-ing parameters recovered from the new model were attributed to the structural change of HAS and its biological ac-tivity. The results obtained indicate that there is a set of two identical binding sites for Er3+ ions with negative co-operativity. The enhancement of complex formation by Er3+ and concomitant increase in △S suggest that the metal ion plays a role in increasing the number of hydrophobic contacts. The binding parameters discovered from the ex-tended solvation model indicate that the stability of HAS molecule is increased as a result of its interaction with Er3+ ions.
作 者: BEHBEHANI G Rezaei DIVSALAR A SABOURY A. A FARIDBOD, F GANJALI M R 作者單位: BEHBEHANI G Rezaei(Chemistry Department,Imam Khomeini International University,Qazvin,Iran)DIVSALAR A(Institute of Biochemistry and Biophysics,University of Tehran,Tehran,Iran;Department of Biological Sciences,Tarbiat Moallem University,Tehran,Iran)
SABOURY A. A(Institute of Biochemistry and Biophysics,University of Tehran,Tehran,Iran)
FARIDBOD, F,GANJALI M R(Departments of Chemistry,University of Tehran,Tehran,Iran)
刊 名: 中國化學(xué)(英文版) SCI 英文刊名: CHINESE JOURNAL OF CHEMISTRY 年,卷(期): 2009 27(2) 分類號: O6 關(guān)鍵詞: human serum albumin isothermal titration calorimetry erbium(Ⅲ) chloride binding site【A High Performance Theory for Thermo】相關(guān)文章:
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