A novel human gene spindlin1,encoding a protein localized in the cell nucleus and inducing NIH3T3 cells transformation

A novel human gene, spindlin1, recently cloned in our laboratory, is highly expressed in the tissue of ovary cancer. To study its biological function, a vector expressing green fluorescent-spindlin1 fusion protein was constructed and transfected into COS-7 and NIH3T3 cells by lipofectamine methods. The results showed that the fusion protein pEGFP-N1-spindlin1 was localized in the nucleus of COS-7 and NIH3T3 cells. NIH3T3 cells which could stably express spindlin1 as a result of RT-PCR analysis compared with the parental NIH3T3 cells displayed a complete morphological change, improved the cell growth and increased the percentage of cells in G2/M phase (12.6% vs control cells at 3.4%). Furthermore, overexpressed spindlin1 cells formed colonies in soft agar, more motile in migration assay in vitro and formed tumors in nude mice. Our findings provide direct evidence that spindlin1 gene may be a prooncogene which is associated with tumorigenesis.

作 者： Gao Yanhong QIN Lipeng ZHANG Peng CHEN Lin YUAN Hongfeng BAI Cixian YAN Fang YUE Wen Pei Xuetao   作者單位： Department of Stem Cell Biology, Beijng Institute of Transfusion Medicine, Beijing 100850, China  刊 名： 自然科學進展(英文版)  SCI 英文刊名： PROGRESS IN NATURAL SCIENCE  年，卷(期)： 2004 14(11)  分類號： Q2  關(guān)鍵詞： spindlin1   gene transfection   subcellular localization   tumorigenesis  

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